rap1 gene  (Medicago)

Journal: bioRxiv

Article Title: Chromosome end protection by RAP1-mediated inhibition of DNA-PK

doi: 10.1101/2024.12.28.630583

Figure Lengend Snippet: a. Outline of DNaseI footprinting experiment. 32 P-labelled 5’ end highlighted by a red asterisk. Radiolabelled template is incubated with KU and DNAPKcs prior to the addition of Shelterin composed of TRF1, TRF2, RAP1, TIN2, POT1 and TPP1. DNase I digested products are then analysed by denaturing Urea-PAGE b-f. DNaseI footprinting of telomere end-binding complexes. Proteins omitted as indicated. Nucleotides from the 5’ telomeric end indicated.

Article Snippet: The shelterin genes (TERF1, TERF2, TERF2IP/RAP1, TINF2, ACD/TPP1, POT1), codon-optimised for E. coli , were synthesised by GenScript and cloned into pACEBAC1, using a nicking cloning system .

Techniques: Footprinting, Incubation, Binding Assay

Journal: Vaccines

Article Title: Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing Plasmodium berghei MSP-8, MSP-9 and RAP1

doi: 10.3390/vaccines10020203

Figure Lengend Snippet: VLPs characterization. MSP-8 ( A ), MSP-9 ( B ) and RAP1 ( C ) VLP morphologies were visualized under TEM. MSP-8 ( D ), MSP-9 ( E ) and RAP1 ( F ) VLPs were loaded (8, 40 µg) for SDS-PAGE, and bands corresponding to each antigen were observed using either the polyclonal P. berghei antibody or the monoclonal M1 antibody.

Article Snippet: Codon-optimized P. berghei rhoptry-associated protein 1 (RAP1) gene was acquired from GenScript (Piscataway, NJ, USA).

Techniques: SDS Page

Journal: Vaccines

Article Title: Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing Plasmodium berghei MSP-8, MSP-9 and RAP1

doi: 10.3390/vaccines10020203

Figure Lengend Snippet: Experimental schedule. Mice were immunized twice with the combined VLP vaccines expressing MSP-8, MSP-9, and RAP1 of P. berghei at 4 weeks intervals, and blood samples were drawn 3 weeks after each immunization. Challenge infection was performed 4 weeks after boost immunization and half of the mice in each group were sacrificed at 1 week post-challenge.

Article Snippet: Codon-optimized P. berghei rhoptry-associated protein 1 (RAP1) gene was acquired from GenScript (Piscataway, NJ, USA).

Techniques: Expressing, Infection

Journal: Vaccines

Article Title: Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing Plasmodium berghei MSP-8, MSP-9 and RAP1

doi: 10.3390/vaccines10020203

Figure Lengend Snippet: IgG antibody response. Mice were intramuscularly immunized twice with the mixture of MSP-8, MSP-9 and RAP1 VLPs. Sera ( n = 6) were collected at regular intervals after each immunization with the VLPs. VLPs immunization induced IgG antibodies reacted with P. berghei Ag ( A ) and VLPs ( B ) upon prime and boost compared to naïve sera (** P < 0.01, *** P < 0.001).

Article Snippet: Codon-optimized P. berghei rhoptry-associated protein 1 (RAP1) gene was acquired from GenScript (Piscataway, NJ, USA).

Techniques:

Journal: Vaccines

Article Title: Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing Plasmodium berghei MSP-8, MSP-9 and RAP1

doi: 10.3390/vaccines10020203

Figure Lengend Snippet: Immune cell responses. Gating strategy for CD4 + , CD8 + T cells and memory B cells ( A ). Numbers of CD4 + ( B ) and CD8 + ( C ) T cells and memory B cells ( D ) were analyzed by flow cytometry after P. berghei challenge infection. Higher numbers of CD4 + T cells ( B , * P < 0.05) and memory B cells ( D , * P < 0.05) were observed from the spleens of VLP immunization group compared to non-immunized control ( n = 6).

Article Snippet: Codon-optimized P. berghei rhoptry-associated protein 1 (RAP1) gene was acquired from GenScript (Piscataway, NJ, USA).

Techniques: Flow Cytometry, Infection

Journal: Vaccines

Article Title: Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing Plasmodium berghei MSP-8, MSP-9 and RAP1

doi: 10.3390/vaccines10020203

Figure Lengend Snippet: Parasitemia and survival. RBCs of P. berghei challenge-infected mice were collected for parasite enumeration via flow cytometry ( A ), ** P < 0.01. Body weight changes and survival rates of mice were determined at two days interval after P. berghei infection ( B , C ). The error bars represent SEM.

Article Snippet: Codon-optimized P. berghei rhoptry-associated protein 1 (RAP1) gene was acquired from GenScript (Piscataway, NJ, USA).

Techniques: Infection, Flow Cytometry